Thp1 nfkb reporter

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August undefined, 2024

WebCL-1641. Description. THP-1/IFN beta reporter (GFP) cells are derived from the human monocytic THP-1 cell line by stably integration of a IFN beta GFP reporter construct. The … WebMay 24, 2024 · Sensing of pathogens by innate immune cells is essential for the initiation of appropriate immune responses. Toll-like receptors (TLRs), which are highly sensitive for …

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WebAim: To study the effects of alpha-pinene on nuclear translocation of nuclear factor-kappa B (NF-kappa B) and the expression of the inhibitor of NF-kappa B (I kappa B alpha) in human monocyte THP-1 cell line. Methods: THP-1 cells were incubated with alpha-pinene (1, 10, and 100 mg/L, for 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg/L, … WebDec 29, 2024 · immortalized bone-marrow-derived macrophages with an NFKB reporter and Cas9 (iBMDM-NFKB-Cas9) Covarrubias et al., 2024. Covarrubias S. Robinson E.K. Shapleigh B. Vollmers A. Katzman S. Hanley N. Fong N. ... Total cellular RNA from THP1 cell lines was isolated using the Direct-zol RNA MiniPrep Kit ... blush pink satin clutch bag

Buy THP1-Lucia™ NF-κB Cells Axil Scientific

WebIFNAR2 knockout NF-κB-SEAP and IRF-Lucia luciferase reporter monocytes. THP1-Dual ™ KO-IFNAR2 cells were generated from THP1-Dual ™ cells by stable knockout of the … WebNFKB-Luc Reporter Cell Line - THP-1. Cat.No. CSC-RR0497. Description. This cell line is derived from THP-1 and is engineered to stably overexpress luciferase reporter gene under the NF-kB response element. WebTHP1-Lucia™ were derived from the human THP-1 monocyte cell line by stable integration of an NF-κB-inducible Luc reporter construct. As a result, THP1-Lucia™ cells allow the … blush pink shell chair

Technical Data Sheet: THP-1 NF-κB-LUC2 - ATCC

WebFurther, chlorojanerin at 5 M also inhibited the binding of NF- B in a GFP reporter assay system by 55.5% thus validating the microarray gene expression data. This work is a step towards the isolation and characterization of lead anti-inflammatory agents from the extract of Saussurea heteromalla, which can be developed into better therapeutic molecules … WebThe TLR4/MD-2/CD14 reporter cell line is a stably co-transfected cell line which expresses full-length human Toll-like receptor 4 (TLR4), human MD-2, human CD14 and secreted alkaline phosphatase (SEAP) reporter genes under the transcriptional control of an NF-kB response element. Cell line are blasticidin, puromycin, zeocin and G418. cleveland cliffs aktie forumWebNFKB-Luc Reporter Cell Line - THP-1. Cat.No. CSC-RR0497. Description. This cell line is derived from THP-1 and is engineered to stably overexpress luciferase reporter gene … blush pink sheepskin rug

"WebNF-κB SEAP Reporter Monocytes. This website uses cookies to ensure the best experience. By continuing, you agree to our " - Thp1 nfkb reporter

Thp1 nfkb reporter

THP-1 Cell-based Assays Monocyte Reporter Cell Lines - InvivoGen

WebAdd to Cart. NanoLuc® Reporter Vector with NF-κB Response Element. 20μg. $ 435.00. Your price: Log in. The pNL3.2.NF-κB-RE [ NlucP /NF-κB-RE/Hygro] Vector contains five copies of an NF-κB response element (NF-κB-RE) that drives transcription of a destabilized form of NanoLuc® luciferase, an engineered small (23.3kDa) luciferase fusion ... WebLPS dose response in NF-κB reporter (Luc)-THP-1 cells. The results are shown as fold induction of luciferase reporter expression. Fold induction was determined by comparing …

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WebOct 9, 2014 · O35379 17250 Abcc1 gene Mus musculus 10090 reporter gene assay evidence ECO:0000049 negative regulation of gene expression GO:0010629 y y PMID:14573776 p53 source not identified Jodi P46531 4851 NOTCH1 protein Homo sapiens 9606 Protein complex Q06330 3516 RBPJ protein Homo sapiens 9606 P33527 4363 … WebThe NF-kappaB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-kappaB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-kappaB response element located upstream of the

WebThis luciferase reporter cell line was derived from parental line TIB-202 by stably expressing firefly luciferase gene (luc2) under control of a NF- κB promoter through lentiviral … Weballele-specific effect was augmented when the cells were treated with the NFkB activator lipopolysaccharides or co-transfected with p50 and/or p65 expressing plasmids, but was reduced when the cells were treated with the NFkB inhibitor 6-Amino-4-(4-phenoxyphenylethylamino)-quinazoline or transfected with a dominant negative mutant of …

WebApr 18, 2024 · InvivoGen 에서는 innate immunity-specific signaling pathway 연구를 위한 가장 다양한 cell line을 공급하고 있습니다. SEAP(secreted embryonic alkaline phosphatase) 혹은 Luciferase가 단독 혹은 동시에 발현되며, 세포 밖으로 분비되는 결과를 측정하게 됩니다. 간단하고 쉽게 detection을 하기 위한 reagent를 개발하여 함께 제공하고 ... WebNF-kB-SEAP and IRF-Lucia luciferase Reporter Monocytes. THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter …

WebInvivoGen thp1 dual tm monocyte cell line Thp1 Dual Tm Monocyte Cell Line, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

WebJun 10, 2016 · Plasmids VectorsPlasmids encoding p65, MAVS, pcDNA3.1 vector, GFP, pCherry,were property ourlab wereall CMVpromoter- driven vectors. pNiFty2NF-kB luciferase reporter (Invivogen) construct secondaryassays PP1-cactivity, additionalPP1-c plasmid obtainedfrom Origene, determineintegrity. furtherused catalyticallyinactive mutant (PP1-c … blush pink sequin fabricWebTHP1 cells were treated with palmitate for different time points and cell lysates were ... (200 um), TNF-α (10 ng/ml) and 0.1% BSA for 24 hrs. SEAP reporter activity (degree of NF-κB /AP-1 ... blush pink sheet setWebNov 26, 2024 · Reporter cell lines were obtained from InvivoGen, including HEK-Blue mTLR7 (hkb-mtlr7), HEK-Blue hTLR8 (hkb-htlr8), and THP1-Lucia NF-kB cells (thp1-nfkb). Cells were maintained following manufacturer's protocols; assays were performed at passage 5-8 for HEK-Blue mTLR7 cells and before passage 12 for HEK-Blue hTLR8 cells to maintain … cleveland cliffs address clevelandWebIRF3 knockout dual reporter monocytes. Nucleic acid sensing signaling in THP1-Dual™ KO-IRF3 cells. THP1-Dual™ KO-IRF3 cells were generated from the THP1-Dual™ cell line, … cleveland cliffs agricultureWeb1700 To better understand molecular bases of MDS pathogenesis, we performed a genome-wide CHIP-Seq analysis of H3K4me3, a histone mark associated with gene activation. In MDS CD34+ cells (N=4), 36 genes showed higher levels of promoter-H3K4me3 compared to controls. 10 of 11 randomly selected genes from these 36 showed increased mRNA … blush pink sage green and grey color schemeWebNov 5, 2024 · Human monocytic THP1 cells with stable overexpression of MD2, CD14 and a NF-κB secreted embryonic alkaline phosphatase (SEAP) reporter construct (THP1-MD2 … cleveland cliffs acquires arcelormittal usaWeb1 day ago · In brief, the assays were host cell adherence with the A549 cell line, host cell toxicity with the THP1 cells, immune activation with the Jurkat NF-kB reporter cells and antibiotic resistance with ... blush pink shoes and bag