WebJan 17, 2024 · M, N (M) Western blot of ZFP207, OCT4, NANOG, and SOX2 in shScr, sh1 and sh2 ESCs and (N) RT-qPCR analysis of Oct4, Nanog, and Sox2 in shScr, sh1 and sh2 ESCs; data is relative to shScr. O, P (O) Western blot of OCT4 during a 9-h cycloheximide (CHX) time course treatment in shScr, sh1 and sh2 ESCs. (P) Protein degradation curves were … Web162 (Fig. 3F). We then investigated the migratory and invasive capacity of shScr and shMCU cells. 163 A profound reduction of approximately 80% in the migratory capacity of shMCU and siMCU 164 cells compared to controls was seen (Fig. 3G-H and Supplementary Fig. 6). MCU knockdown
Addgene: PLKO-mcherry-luc-puro-shSCR
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