Phospho-akt thr308

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August undefined, 2024

Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor ... WebThe Phospho-Akt (Thr308) Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-Akt (Ser308) and total Akt in human, mouse, and rat whole cell lysates. Specifications Kit Contents Documentation References

HTRF PHOSPHO AKT DETECTION KITS - PerkinElmer

WebNational Center for Biotechnology Information WebThe Phospho-AKT (Thr308) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the … cyst on stomach area

Phospho-AKT (Thr308) Monoclonal Antibody (18F3.H11), Biotin

WebSep 12, 2015 · All Aktisoforms undergo membrane recruitment from cytoplasmthrough pleckstrinhomology domain membranePtdIns(3,4,5)P leadingAkt activation Thr308 Ser473 membrane-associatedkinases (14, 15). kinaseresponsible Thr308 phosphorylation PtdIns-dependent kinase-1, whereas Ser473 kinase unclear(16, 17). WebAKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. WebPhospho-Akt (Thr308) (D25E6) XP ® Rabbit mAb recognizes endogenous levels of Akt1 protein only when phosphorylated at Thr308. This antibody also recognizes endogenous … cyst on stretch mark

Comparing PI3K/Akt Inhibitors Used in Ovarian Cancer Treatment

Regulation of AKT Phosphorylation at Ser473 and Thr308 by ... - PLOS

Webhamster AKT 1/2/3 (Thr308) in cell lysates. Detection of rat AKT 1/2/3 (Thr308) is also expected. The InstantOne ELISA™ kit allows for fast analysis of samples in approximately … WebPhospho (Thr308, Thr309, Thr305) View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt. binding of isaac mort babyWebPhospho-AKT (Thr308) Monoclonal Antibody (18F3.H11) (200-301-269) Popular TaqMan Real-Time PCR Assays Antibodies Oligos, Primers & Probes GeneArt Gene Synthesis Cell Culture Plastics Applications & … cyst on tailbone surgery recovery

"WebMar 3, 2024 · Phospho-Akt (p-Akt) on Ser473 (S473), p-Akt on Thr308 (T308), and Akt protein expression in cancerous tissues and survival analysis of ovarian cancer patients were directly obtained from The Cancer Proteome Atlas (TCPA) database 1 … " - Phospho-akt thr308

Phospho-akt thr308

Regulation of AKT Phosphorylation at Ser473 and Thr308 by

WebThis Anti-phospho-Akt (Thr308) Antibody is validated for use in IH(P) for the detection of phospho-Akt (Thr308). Key Applications: Immunohistochemistry (Paraffin) Applications … WebAnti-phospho-Akt (pThr308); Anti-phospho-Akt (pThr308) antibody produced in rabbit has been used in immunoblotting. US EN. Applications Products Services Support. …

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WebApr 5, 2024 · Akt activation can lead to the phosphorylation of RXRXX(S/T) sequences of transcription factors, controlling apoptosis or pro-survival molecules [10]. For instance, PI3K/Akt activation by NTFs mediates in vitro MN survival, whereas their inhibition causes apoptotic cell death [11, 12]. WebHK2 isoform is under physiological conditions predominantly localized in the cytosol and upon stimulation of PI3K/ Akt pathway associates with mitochondria and thus can …

WebThe Phospho-Akt (Thr308) Antibody was generated using AKT1, AKT2, and AKT3 as the antigen and it reacts with Human, Mouse, and Rat. Phospho-Akt (Thr308) Antibody detects endogenous levels of Akt only when phosphorylated at Thr308 Phospho-Akt (Thr308) Antibody Supplier Page Product Specs Company Info Citations Reviews Product Specs WebAntibodies and Reagents —Antibodies employed include the following: anti-phospho-Akt Ser-473, anti-phospho-Akt Thr-308, anti-phospho-ERK1/2 (Thr-202/Tyr-204), anti-HA, pan-anti-phospho- (Ser/Thr) Akt substrate, anti-phospho-FoxO1/3a (Thr-24/Thr-32), and anti-PP2A/B from Cell Signaling; and anti-PP2A/A from Santa Cruz Biotechnology; anti-PP2A/B …

WebJan 22, 2009 · AML patients were stratified into two groups, Thr308 high or Thr308 low, according to the value of phospho-Akt Thr308 (rMFI >2.3, Thr308 high and rMFI ⩽ 2.3, Thr308 low). WebPhospho-AKT (Thr308) Antibody (600-401-269) in IHC Immunohistochemistry of Rabbit Anti-AKT pT308 Antibody. Tissue: human testis tissue. Antigen retrieval: Heat induced …

WebPhospho-Akt (Thr308) Whole Cell Lysate Kit. The Phospho-Akt (Thr308) Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho … binding of isaac mother\u0027s braceletWebMar 21, 2011 · Anti-p70 S6 kinase, Anti-phospho-AKT (Thr308), Anti-AKT1, and anti-GRP78 (N-20) were from Santa Cruz Biotechnologies (Insight Biotechnology Ltd, UK). Anti-HSP70 … binding of isaac movement bugWebNov 7, 2015 · Antibodies against total phospho-AMPK(Thr172), total phospho-ACC(Ser79), total phospho-p53(Ser15), phospho-Raptor (Ser722/ Ser792), total phospho-mTOR(Ser2448), total phospho-p70S6K(Thr389), total phospho4EBP-1 (Thr 37/46), total phospho-Akt(Ser 473), LC3 I/II β-actinwere obtained fromCell Signaling Technology, Danvers, MA. mTOR … cyst on tendon on bottom of footWebImmunofluorescent analysis of Phospho-AKT pThr308 in serum-starved HeLa cells treated with insulin (100 ng/mL, 15 min) using a Phospho-AKT pThr308 recombinant rabbit monoclonal antibody ( Product # 701052) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). binding of isaac mouse padWebwavelength (665 nm). The specific signal modulates positively in proportion to phospho-AKT (Thr308). The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of phospho-AKT (Thr308) by HTRF ® reagents. binding of isaac mr meWebThis Anti-phospho-Akt (Thr308) Antibody is validated for use in IH (P) for the detection of phospho-Akt (Thr308). Anti-phospho-Akt (Thr308) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS CoA binding of isaac moving boxWebthese cells. Silencing of B55 phatase (PHLPP) (17) was reported to regulate Akt activity.expression markedly increased phosphorylation at Thr-308 but not at Ser-473 in both FL5.12 cells and NIH3T3 cells. Consistently, PP2A AB55 C holoen-zymes preferentially dephosphorylated phospho-Thr-308 rather than phospho-Ser-473 in in vitro ... cyst on tailbone symptoms