Phospho 4ebp1 antibody

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August undefined, 2024

WebMar 30, 2016 · When 4EBP1, an eIF4E-binding protein, is active (non-p-4EBP1), it binds to eIF4E and hinder formation of the initiation complex and then translation is blocked, favoring apoptosis.... WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the …

Phospho-4EBP1 (Thr37/46) cellular HTRF kit Cisbio - PerkinElmer

WebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent ... Web丁香通为您提供磷酸化4e结合蛋白1抗体商品详情介绍：价格：询价，货号：qy-m3780，品牌：乔羽，产地：进口/国产，保存条件 ... crystal gateway marriott alexandria

Phosphorylated 4EBP1 is associated with tumor progression and …

WebNov 11, 2008 · To determine whether CDKs could be responsible for the rapamycin-stimulated rephosphorylation of 4EBP1, we treated cells with saturating concentrations of roscovitine (50 μM), a CDK inhibitor. ... Phospho-S6 and actin were blotted for normalization. (D) The experiments were completed in the same way as in C, except WT 4E-BP1, AA 4E … WebJun 25, 2010 · Anti-phospho Drosophila S6K, anti-S6K, anti-phospho S6K, anti-Akt, anti-phospho Akt, and anti-phospho 4EBP1 antibodies were from Cell Signaling. Anti-Myc, anti-HA, and anti-FLAG antibodies were from Santa Cruz Biotechnology, Covance, and Sigma, respectively. ... stimulation for 30 min. Phosphorylation and protein levels of dS6K were … WebInvitrogen Anti-Phospho-4EBP1 (Thr37, Thr46) Polyclonal, Catalog # PA5-114544. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry (PFA fixed) (IHC (PFA)) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL). dwd new york

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WebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes WebFeb 10, 2024 · Cell Signaling Technology's Phospho-4E-BP1 (Thr70) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. Phospho-4E-BP1 (Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70. crystal gateway marriott airport parkingWebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. crystal gateway marriott arlington

"WebPhospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence. " - Phospho 4ebp1 antibody

Phospho 4ebp1 antibody

Phospho-4EBP1 (Thr37, Thr46) Polyclonal Antibody

WebThis antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1. Tested applications Suitable for: IHC-P, WB more details Species reactivity Reacts with: Rat, Human Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan Immunogen WebJun 12, 2014 · Total 4EBP1 antibody detects both phosphorylated and non-phosphorylated protein. Samples were treated with lambda-phosphatase or reaction buffer alone and decrease in phosphorylation was noted. X-axis represents iso-electric pH and y-axis represents luminescence units.

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WebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). Species … WebMA Beigh's Publication in Biomed Res Int....Sigma unless otherwise indicated. Anti-S6K antibody (Santa Cruz Biotechnologies Inc), Anti-S6K (Polyclonal-Genscript), Anti-Erk1, Anti-phospho-Erk, Anti-4EBP1, Anti-phospho- 4EBP1-T70 and Anti-phospho-S6K Thr412 antibodies were obtained from Cell Signaling...

WebAll lanes : Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate Lane 2 : HeLa treated with 100nM rapamycin for 24 hours, whole cell whole cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at … WebFigure 3: Anti-4E-BP1 (phospho Ser65 + Thr70) Antibody (AF2308) Western blot analysis of extracts from 293 cells treated with 20% FBS , using Phospho-4EBP1(Ser65/Thr70) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.

WebAnti-eIF4EBP1 (phospho T36) antibody See all eIF4EBP1 primary antibodies Description Rabbit polyclonal to eIF4EBP1 (phospho T36) Host species Rabbit Specificity ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. WebPhospho-4EBP1 (Thr37, Thr46) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. View all (63) 4EBP1 antibodies Add To Cart Save to list Performance Guarantee Datasheet Protocols Questions & Answers Tech Support Antibody Testing Data (1)

WebAnti p-eIF4EBP1 (Ser65/Thr70) antibody, Anti BP1 antibody, Anti BP 1 antibody, Anti eIF4EBP1 (phospho Ser65/Thr70) antibody, Anti PHAS I antibody, Anti 4EBP1 antibody, Anti PHASI antibody, Anti 4E-BP1 antibody, Anti eIF4E-binding protein 1 antibody, Anti eIF4EBP1 (phospho S65/Th70) antibody, Anti 4E BP1 antibody, Anti eIF4E binding protein 1 …

WebThe lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. 溶质携带物家族-1抗体， ... dw/docuware/platform/webclient/ntlmWebOur 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the 4EBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. dwd office indianapolisWebEach 4EBP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. dw documentary islamWebAbcam - antibodies and reagents supplier, find any antibody Hello. We're improving abcam.com and we'd welcome your feedback. ... 4ebp1 35 results for 4ebp1 ... Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] - BSA and Azide free (ab283288) dw documentary - youtubeWebSep 7, 2024 · 4E-BP1 Antibody (P-1) is recommended for detection of 4E-BP1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC (P) and ELISA. Anti-4E-BP1 Antibody (P-1) is available conjugated to agarose for IP; HRP for WB, IHC (P) and ELISA; and to either phycoerythrin or FITC for IF, IHC (P) and FCM. crystal gateway marriott airport shuttleWeb实验用到的供体是Thr46（铽标记的anti-phospho-4EBP1），受体是绿色荧光标记的4EBP1，是mTOR下游信号通路的蛋白，有活性的mTOR能磷酸化4EBP1，使之与供体结合。如果mTOR活性受到抑制，供体受体距离不能达到荧光能量转移的要求，则在515nm处与485nm处的信号比值增大。 crystal gateway marriott - arlington vaWebThe Simple Western TM antibody database is a user-interactive listing of antibodies that have been screened and tested in Simple Western Size- and Charge-based assays. This database is intended to provide general assay development guidance for identifying and selecting antibodies to test. crystal gateway marriott general manager